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Another study found that treatment with metformin can regulate the immune response in the intestines of mice by altering the composition of the gut microbiome, particularly through the downregulation of the NF-κB signaling pathway ( Metformin has been shown to have some anti-inflammatory effects in clinical studies in patients with T2DM, in experimental studies in rodent models of obesity and T2DM, and in vitro experiments with several immune cells (Kristófi and Eriksson, 2021)