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To mitigate this issue, a SsrA degradation tag was integrated into the Npu SICLOPPS inteins, enabling the bacterial protease ClpXP to degrade the spliced inteins

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FIGURE 10 Notably, SR18 also contains a potential trypsin cleavage site (Figure 10), and the HRMS data (Supplementary Figure S7) with a peak around ∼685 Da confirm that trypsin can cleave SR18 between Lys17 and Ala18, leaving the remaining crucial 17 residues intact