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ELISA Kit Components Required Instruments and Reagents Microplate reader (wavelength: 450nm) 37°C incubator (CO2 incubator for cell culture is not recommenced.) Automated plate washer or multi-channel pipette/5ml pipettor (for manual washing purpose) Precision single (0.5-10μL, 5-50μL, 20-200μL, 200-1000μL) and multi-channel pipette with disposable tips(Calibration is required before use.) Sterile tubes and Eppendorf tubes with disposable tips Absorbent paper and loading slot Deionized or distilled water Assay Procedure Summary Step 1: Add 100ul standard or sample into each well, seal the plate and statically incubate for 90 minutes at 37°C