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For immunofluorescence, the primary antibodies used were monoclonal [8G9] to GLP-1, monoclonal SST antibody (#MAB2358), and monoclonal Human/Mouse Ghrelin Antibody (R&D systems #MAB8200), and the secondary antibodies were Alexa Fluor® 488 AffiniPure Donkey Anti-Rat IgG and Cyanine Cy™3 AffiniPure Donkey Anti-Mouse IgG from Jackson ImmunoResearch (Europe Ltd.) and were used at a final dilution of 1/300

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15 Consistent with these findings, a large post hoc analysis of the STEP 1, 2, 3 and 5 trials reported a statistically significant but clinically negligible reduction in depressive symptoms among individuals without major psychiatric illness treated with semaglutide (estimated treatment difference: −0.56