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qPCR assays SPHKAP knock-down efficiency Mouse islets transduced with either Control or SPHKAP shRNA lentiviral particles were lysed using Trizol and RNA extracted using 200 ฮผL chloroform per 1 mL Trizol and then precipitated in 500 ฮผL isopropanol and 5 mg glycogen per mL of Trizol
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