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Human embryonic kidney 293 cells containing SV40 large T-antigen (HEK293T) were cultured in DMEM (Gibco) supplemented with 10% (v/v) fetal bovine serum (FBS, Gibco), 1 mM sodium pyruvate (Gibco) and 100 units/mL penicillin and 100 μg/mL streptomycin at 37 °C in 5% CO 2
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Each patient receives enough time to describe their challenges, goals, and previous attempts, and this information becomes the foundation for a personalized treatment plan
